Journal: Scientific Reports

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1038/s41598-019-45798-5

Figure Lengend Snippet: RAGE inhibition restores alveolar fluid clearance after acid-induced lung injury. Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. P = 0.02 for the overall multiple group test; no statistical difference was found in post-hoc tests.

Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

Techniques: Inhibition, Marker, Recombinant, Whisker Assay

Journal: Scientific Reports

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1038/s41598-019-45798-5

Figure Lengend Snippet: RAGE inhibition improves arterial oxygenation after acid-induced lung injury. Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Individual values are reported. Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

Techniques: Inhibition, Recombinant

Journal: Scientific Reports

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1038/s41598-019-45798-5

Figure Lengend Snippet: RAGE inhibition decreases alveolar-capillary permeability after acid-induced lung injury. Values are reported as box and whisker plots. ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco + , Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group at each time point). Time × group interaction and post-hoc comparisons were verified using random effects models to analyse longitudinal evolution of variables.

Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

Techniques: Inhibition, Permeability, Whisker Assay, Recombinant

Journal: Scientific Reports

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1038/s41598-019-45798-5

Figure Lengend Snippet: RAGE inhibition decreases alveolar inflammation after acid-induced lung injury. Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used.

Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

Techniques: Inhibition, Recombinant, Whisker Assay

Journal: Scientific Reports

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1038/s41598-019-45798-5

Figure Lengend Snippet: (A ) RAGE inhibition decreases histological features of lung injury. Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl + RAP) or recombinant sRAGE (HCl + sRAGE) (n = 12 per group). Values are reported as box and whisker plots. The Kruskal-Wallis test, with post-hoc Bonferroni test for pairwise comparisons were used. (B – E) Representative hematoxylin and eosin–stained sections at x20 original magnification of (B) uninjured piglets (Sham), (C) acid-injured piglets (HCl), acid-injured piglets treated with (D) RAGE antagonist peptide (HCl + RAP) or (E) recombinant sRAGE (HCl + sRAGE). There was greater cellularity consisting mainly of neutrophils (black arrowheads), more alveolar wall thickening (white arrowheads), and more areas of atelectasis and increased alveolar disruption, proteinous debris, and hemorrhage (black arrows) in untreated, acid-injured than in uninjured or treated piglets. Scale bars, 100 µm.

Article Snippet: The “sRAGE group” (n = 12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg −1 ) (Recombinant Human RAGE Fc Chimera, R&D Systems, Minneapolis, MN).

Techniques: Inhibition, Recombinant, Whisker Assay, Staining, Disruption

Journal: bioRxiv

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1101/405423

Figure Lengend Snippet: Measurement of net alveolar fluid clearance (AFC) rate as a marker of epithelial function in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Marker, Recombinant

Journal: bioRxiv

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1101/405423

Figure Lengend Snippet: Arterial oxygen tension (PaO 2 )/inspiratory oxygen fraction (FiO 2 ) in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Recombinant

Journal: bioRxiv

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1101/405423

Figure Lengend Snippet: ( A ) Level of total protein in the bronchoalveolar lavage (BAL) fluid from uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). ( B ) Extravascular lung water, as measured by transpulmonary thermodilution (Picco+, Pulsion SA) and indexed to body weight, in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group at each time point). Values are reported as medians and interquartile ranges.

Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Recombinant

Journal: bioRxiv

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1101/405423

Figure Lengend Snippet: Measurement of bronchoalveolar lavage (BAL) levels of ( A ) tumour necrosis factor (TNF)-α, ( B ) interleukin (IL)-6, ( C ) IL-1β and ( D ) IL-18 in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Values are reported as medians and interquartile ranges.

Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Recombinant

Journal: bioRxiv

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1101/405423

Figure Lengend Snippet: Lung injury scores were higher in acid-injured (HCl) than in uninjured piglets (Sham) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Values are reported as medians and interquartile ranges.

Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Recombinant

Journal: bioRxiv

Article Title: Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets

doi: 10.1101/405423

Figure Lengend Snippet: The gene expression levels of α1-ENaC (NCBI Reference Sequence, NM_213758.2), α1-Na,K-ATPase (NM_214249.1), AQP-5 (NM_001110424.1) and 36B4 (a housekeeping gene) were assessed using the quantitative real-time reverse transcription polymerase chain reaction in uninjured (Sham), acid-injured (HCl) and acid-injured piglets treated with RAGE antagonist peptide (HCl+RAP) or recombinant sRAGE (HCl+sRAGE) (n = 12 per group). Threshold levels of mRNA expression (ΔΔCt) were normalised to the housekeeping gene 36B4 and the values represent the mean of triplicate samples ± SD. Data are representative of three independent observations.

Article Snippet: The “sRAGE group” (n=12) included animals with HCl-induced lung injury that also received intravenous treatment with sRAGE (3 mg.kg -1 ) (RAGE (N-16) peptide, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Gene Expression, Sequencing, Reverse Transcription, Polymerase Chain Reaction, Recombinant, Expressing